|
KMID : 0379119930210040279
|
|
Korean Journal of Mycology
1993 Volume.21 No. 4 p.279 ~ p.284
|
|
|
Cloning and Expression of the metE gene coding for homocysteine methyltransferase from the basidiomycete Ganoderma lucidum in E. coli
|
|
|
|
|
Abstract
|
|
|
|
The metE gene coding for N^5-methyl-H₄-folate; homocysteine methyltransferase from the basidiomycete Ganoderma lucidum was cloned by complementation of methionine-requiring mutants of E. coli. The size of a inserted DNA was about 1.54 kb and had 5 restriction enzyme sites. A physical map was constructed. Southern blot analysis confirmed the presence of a transforming DNA in the genome of Ganoderma lucidum, indicating the presence of a single copy.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|